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anti human il12p70  (R&D Systems)


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    R&D Systems anti human il12p70
    Anti Human Il12p70, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+human+il12p70/Human+IL-12+p70+Antibody/pm39557863-379-6-8
    Average 94 stars, based on 39 article reviews
    anti human il12p70 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: IL-12 drives the expression of the inhibitory receptor NKG2A on human tumor-reactive CD8 T cells.
    Article Snippet: .. For IL-12p70 and CD40L blocking experiments, anti-Human Il12p70 (R&D Systems, cat #MAB219-100, 10 μg/ml final), anti-CD40 (BioLegend, cat #313019, clone HB14, 10 μg/ml final) or isotype control mouse IgG1 (BioLegend, cat #400166, clone MOPC21, 10 μg/ml final) were added in the corresponding wells. ..

    Control:

    Article Title: IL-12 drives the expression of the inhibitory receptor NKG2A on human tumor-reactive CD8 T cells.
    Article Snippet: .. For IL-12p70 and CD40L blocking experiments, anti-Human Il12p70 (R&D Systems, cat #MAB219-100, 10 μg/ml final), anti-CD40 (BioLegend, cat #313019, clone HB14, 10 μg/ml final) or isotype control mouse IgG1 (BioLegend, cat #400166, clone MOPC21, 10 μg/ml final) were added in the corresponding wells. ..



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    Figure 2. Soluble <t>IL12p70</t> from poly(I:C)-matured moDC preferentially induces NKG2A but not KIR on KIRnegNKG2Aneg NK cells. A–D, freshly isolated NK cells were sorted for the absence of KIR and NKG2A receptors (KIRnegNKG2Aneg), then cocultured for 6 days either alone (media) or with LCcyto, moDCcyto, moDCLPS, or moDCpoly, but without any additional cytokines in culture. A neutralizing antibody to soluble IL12p70 (þanti-IL12) or its isotype control (þIgG1) was added to moDCpoly cocultures. After 6 days of stimulation, these KIRnegNKG2Aneg NK cells were assayed for NKG2A (A and B) or KIR (C and D) expression, with representative flow cytometric contour plots from the same experimental sample shown. Bar graphs depict pooled phenotypic data from all experiments (mean SD). Asterisks above each error bar refer to the statistical difference between that condition and the unstimulated dþ6 KIRnegNKG2Aneg NK cells (media), whereas statistical notations above the brackets refer to the indicated comparisons between conditions. E and F, CTV-labeled KIRnegNKG2Aneg cells were cocultured for 6 days either alone (media) or with moDCpoly to correlate proliferation with NKG2A (E) or KIR (F) induction. Proliferation was measured by the dilution in fluorescent intensity of CTV. One representative experiment out of three is shown. , P ¼ 0.001–0.01; , P < 0.001; , P 0.0001; ns, not statistically significant.
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    Figure 2. Soluble <t>IL12p70</t> from poly(I:C)-matured moDC preferentially induces NKG2A but not KIR on KIRnegNKG2Aneg NK cells. A–D, freshly isolated NK cells were sorted for the absence of KIR and NKG2A receptors (KIRnegNKG2Aneg), then cocultured for 6 days either alone (media) or with LCcyto, moDCcyto, moDCLPS, or moDCpoly, but without any additional cytokines in culture. A neutralizing antibody to soluble IL12p70 (þanti-IL12) or its isotype control (þIgG1) was added to moDCpoly cocultures. After 6 days of stimulation, these KIRnegNKG2Aneg NK cells were assayed for NKG2A (A and B) or KIR (C and D) expression, with representative flow cytometric contour plots from the same experimental sample shown. Bar graphs depict pooled phenotypic data from all experiments (mean SD). Asterisks above each error bar refer to the statistical difference between that condition and the unstimulated dþ6 KIRnegNKG2Aneg NK cells (media), whereas statistical notations above the brackets refer to the indicated comparisons between conditions. E and F, CTV-labeled KIRnegNKG2Aneg cells were cocultured for 6 days either alone (media) or with moDCpoly to correlate proliferation with NKG2A (E) or KIR (F) induction. Proliferation was measured by the dilution in fluorescent intensity of CTV. One representative experiment out of three is shown. , P ¼ 0.001–0.01; , P < 0.001; , P 0.0001; ns, not statistically significant.
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    Figure 2. Soluble IL12p70 from poly(I:C)-matured moDC preferentially induces NKG2A but not KIR on KIRnegNKG2Aneg NK cells. A–D, freshly isolated NK cells were sorted for the absence of KIR and NKG2A receptors (KIRnegNKG2Aneg), then cocultured for 6 days either alone (media) or with LCcyto, moDCcyto, moDCLPS, or moDCpoly, but without any additional cytokines in culture. A neutralizing antibody to soluble IL12p70 (þanti-IL12) or its isotype control (þIgG1) was added to moDCpoly cocultures. After 6 days of stimulation, these KIRnegNKG2Aneg NK cells were assayed for NKG2A (A and B) or KIR (C and D) expression, with representative flow cytometric contour plots from the same experimental sample shown. Bar graphs depict pooled phenotypic data from all experiments (mean SD). Asterisks above each error bar refer to the statistical difference between that condition and the unstimulated dþ6 KIRnegNKG2Aneg NK cells (media), whereas statistical notations above the brackets refer to the indicated comparisons between conditions. E and F, CTV-labeled KIRnegNKG2Aneg cells were cocultured for 6 days either alone (media) or with moDCpoly to correlate proliferation with NKG2A (E) or KIR (F) induction. Proliferation was measured by the dilution in fluorescent intensity of CTV. One representative experiment out of three is shown. , P ¼ 0.001–0.01; , P < 0.001; , P 0.0001; ns, not statistically significant.

    Journal: Cancer Immunology Research

    Article Title: Phenotypic and Functional Activation of Hyporesponsive KIRnegNKG2Aneg Human NK-Cell Precursors Requires IL12p70 Provided by Poly(I:C)-Matured Monocyte-Derived Dendritic Cells

    doi: 10.1158/2326-6066.cir-14-0054-t

    Figure Lengend Snippet: Figure 2. Soluble IL12p70 from poly(I:C)-matured moDC preferentially induces NKG2A but not KIR on KIRnegNKG2Aneg NK cells. A–D, freshly isolated NK cells were sorted for the absence of KIR and NKG2A receptors (KIRnegNKG2Aneg), then cocultured for 6 days either alone (media) or with LCcyto, moDCcyto, moDCLPS, or moDCpoly, but without any additional cytokines in culture. A neutralizing antibody to soluble IL12p70 (þanti-IL12) or its isotype control (þIgG1) was added to moDCpoly cocultures. After 6 days of stimulation, these KIRnegNKG2Aneg NK cells were assayed for NKG2A (A and B) or KIR (C and D) expression, with representative flow cytometric contour plots from the same experimental sample shown. Bar graphs depict pooled phenotypic data from all experiments (mean SD). Asterisks above each error bar refer to the statistical difference between that condition and the unstimulated dþ6 KIRnegNKG2Aneg NK cells (media), whereas statistical notations above the brackets refer to the indicated comparisons between conditions. E and F, CTV-labeled KIRnegNKG2Aneg cells were cocultured for 6 days either alone (media) or with moDCpoly to correlate proliferation with NKG2A (E) or KIR (F) induction. Proliferation was measured by the dilution in fluorescent intensity of CTV. One representative experiment out of three is shown. , P ¼ 0.001–0.01; , P < 0.001; , P 0.0001; ns, not statistically significant.

    Article Snippet: In selected NK:DC coculture experiments, soluble IL12p70 was blocked using a neutralizing anti-IL12p70 mAb (clone 24910; R&D Systems).

    Techniques: Isolation, Control, Expressing, Labeling